Determination of iodine in workplace air by inductively coupled plasma mass spectrometry / 中国职业医学

Objective To establish a solvent desorption inductively coupled plasma-mass spectrometry (ICP-MS) method for determination of iodine in workplace air. Methods Iodine in workplace air was collected with alkaline activated carbon tube and desorbed with 10.0 mL pure water or 20 mmol/L sodium bicarbonate solution. Rhenium-185 was used as an internal standard for quantification. The sample was determined in standard mode and kinetic energy discrimination collision (KED) mode by ICP-MS. Results In standard mode, iodine showed a good linear range in the concentration of 9.0 to 1 100.0 μg/L, with a correlation coefficient of 0.999 3 and a detection limit of 2.7 μg/L. In KED mode, iodine showed a good linear range in the concentration of 24.3 to 800.0 μg/L, with a correlation coefficient of 0.999 1 and a detection limit of 7.3 μg/L. The average desorption efficiency using pure water ranged from 99.1% to 106.7%, with within-run relative standard deviation (RSD) of 3.1% to 8.0% and between-run RSD of 4.9% to 9.3%. The average desorption efficiency using sodium bicarbonate solution ranged from 96.5% to 105.3%, with within-run RSD of 4.9% to 8.6% and between-run RSD of 2.5% to 9.9%. There were no statistical significant differences in the main effects of desorption solution, ICP-MS detection mode, their interaction on average desorption efficiency and within-run RSD (all P>0.05). Samples could be stored at room temperature for at least 7 days. Conclusion This method is highly sensitive, accurate, and suitable for the determination of iodine in workplace air. The sample pretreatment is simple and rapid.

PubMed-based establishment of classification for papers on research of basic and clinical medicine / 中华医学图书情报杂志

The classification for papers on research of basic and clinical medicine was studied using the MeSH and check tags indexing rules according to the NIH definition of research on clinical medicine based on PubMed, and its scientificalness was verified according to the papers published in New England Journal of Medicine, Lancet, and Journal of American Medical Association from 2006 to 2015. The classification is of realistic significance for the scientific research management departments to keep abreast of the status quo and level of research on basic and clinical medi-cine and to make policy of scientific research management.

Detecting zirconium and its compounds in workplace air by inductively coupled plasma-mass spectrometry / 中国职业医学

OBJECTIVE: To establish a detection method for zirconium and its compounds in the workplace air with inductively coupled plasma-mass spectrometry( ICP-MS). METHODS: The samples in workplace air were collected by micro filtration membrane. The water soluble zirconium compounds were eluted with 2. 0 mol / L hydrochloric acid and then pre-digested by nitric acid. The water insoluble zirconium compounds were digested by ammonium sulfate solution and then determined by ICP-MS. RESULTS: There were good linear relationship in the range of 0. 000-1. 000 mg / L, and the correlation coefficient was 0. 999 9. The detection limit was 0. 1 μg / L,and the minimum detection concentration was 0. 07μg / m~3(sample volume was 75 L). For the water soluble zirconium compounds the average recovery of standard addition was 95. 0 %-98. 8 %. The with-in run relative standard deviation( RSD) was 2. 0 %-3. 9 % and the between-run RSD was2. 8 %-6. 6 %. For the water insoluble zirconium compounds, the average recovery of standard addition was 94. 4 %-98. 7 %. The with-in run RSD was 2. 1 %-4. 2 % and the between-run RSD was 3. 2 %-4. 7 %. The samples could be stored at room temperature for at least 30 days. CONCLUSION: The method is rapid,accurate and high sensitivity. It can be used for quantitative detection of zirconium and its compoundsin workplace air.

Effect of polymorphism of human intestinal fatty acid binding protein gene on the therapeutic efficacy of fenofibrate / 中国医学科学院学报

<p><b>OBJECTIVE</b>To explore the effect of polymorphism in codon Ala54Thr of human intestinal fatty acid-binding protein gene (IFABP) on the therapeutic efficacy of fenofibrate.</p><p><b>METHODS</b>Totally 147 patients with hyperlipidemia [72 men mean age: (56.2 +/- 8.63) years; 75 women mean age: (58.4 +/- 9.12) years] were enrolled. IFABP genotypes were detected by polymerase chain reaction, Hha I digestion, and sequencing. Four weeks before and after treatment, the levels of fasting serum total cholesterol (TC), triglyceride (TG), high density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C), apolipoprotein A I (apoA I) and apolipoprotein B (apoB) were detected with biochemical techniques.</p><p><b>RESULTS</b>The frequency of IFABP genotype was 0.47 for A/A, 0.37 for A/T, and 0.16 for T/T, and the allelic frequency was 0.65 for A and 0.35 for T. No significant different was found in lipid levels in every genotype before treatment (P > 0.05). After 4 weeks of treatment, the levels of TC, TG, LDL-C, and apoB significantly decreased (P < 00.01), and the levels of HDH-C and apoA I significantly increased (P < 0.01). The total therapeutic efficacy on A54A and A54T were 97% and 95%, respectively. In the patients with T54T genotype after treatment, no significant difference in lipids levels was found except TG (P < 0.05), and the total efficacy was only 38%. The total therapeutic efficacies of fenofibrate on A54A and A54T were higher than those of T54T, and there was significant different between A54A and T54T (P < 0.01).</p><p><b>CONCLUSION</b>The polymorphism of human IFABP gene in hyperlipidemia is related with the therapeutic efficacy of fenofibrate, and the T54T IFABP genotype may have strong negative effect on such efficacy.</p>

Malignant transformation of NIH3T3 cells induced by ectopic expression of PC-1 gene / 中华病理学杂志

<p><b>OBJECTIVE</b>To establish a mouse fibroblastic cell line stably transfected with PC-1 gene, and using such cell line to investigate tumor development and progression imposed by the ectopic expression of PC-1 gene.</p><p><b>METHODS</b>Eukaryotic expression vector pcDNA3.1(-)/myc-his-pc-1 was transfected into mouse fibroblast cell line NIH3T3 by lipofectamine. Stable transfectants were selected by G418. The integration and expression of ectopic PC-1 gene were analyzed by PCR and RT-PCR. Cytomorphological analysis, MTT, soft agar colony formation and nude mice tumorigenesis assay were used to evaluate the effects of PC-1 gene expression on tumor development and progression.</p><p><b>RESULTS</b>NIH 3T3 cell lines stably expressing PC-1 gene were successfully established and confirmed by PCR and RT-PCR analyses of the integration and expression of ectopic PC-1 gene. Comparing with the parental cell line and cells transfected with control vector, the PC-1 gene transfectants acquired several phenotypes of transformed cells: increasing growth rate, ability to grow and form cell colonies on soft agar, and becoming tumorigenic in nude mice.</p><p><b>CONCLUSION</b>Ectopic expression of PC-1 gene in NIH3T3 cells can induce malignant transformation of mouse fibroblastic cells both in vitro and in vivo.</p>